Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage
نویسندگان
چکیده
In contrast to decellularization of soft tissues for use as tissue-specific matrices in the creation tissue-engineered constructs, cartilage tissue requires several processing techniques, which can negatively affect biocompatibility and functional properties native extracellular matrix (ECM). Objective: study biocompatible microdispersed 3D from a porcine that is decellularized by sequential chemical, physical enzymatic techniques. Materials methods. For decellularization, particles (MCPs), obtained cryomilling, were incubated detergent solutions (sodium dodecyl sulfate Triton X-100), then treated with supercritical carbon dioxide (scCO2) 10% ethanol DNase I. The Ames test (Salmonella typhimurium reverse mutation assay) was used determine genotoxicity (dMCPs). Local general toxic effects, well resorption dMCPs studied vivo on sexually mature outbred rats. Decellularized MCP specimens (10 mg) implanted into thigh muscle tissue. Viability human adipose-derived mesenchymal stem/stromal cells (hAdMSCs), when cultured dMCPs, analyzed microscopy, stained fluorescent Calcein AM dye. Cell metabolic activity assessed using PrestoBlue™ Reagent. Results. It has been proven rat after treatment scCO2 do not exhibit local show negative effects reproductive system animals. After 6 months experiment, most (87%) resorbed. shown resulting are able support adhesion proliferation hAdMSCs. Conclusion. Porcine dMCP suitable medical products terms toxicity, be creating cell- constructs.
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ژورنال
عنوان ژورنال: Vestnik Transplantologii i Iskusstvennyh Organov
سال: 2022
ISSN: ['2412-6160', '1995-1191']
DOI: https://doi.org/10.15825/1995-1191-2022-4-73-84